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Genotoxicity of environmentally relevant concentrations of water-soluble oil components in cod (Gadus morhua).
Holth, Tor F; Beylich, Bjørnar A; Skarphédinsdóttir, Halldóra; Liewenborg, Birgitta; Grung, Merete; Hylland, Ketil.
Afiliación
  • Holth TF; Norwegian Institute for Water Research (NIVA), CIENS, Gaustadalléen 21, NO-0349 Oslo, Norway.
Environ Sci Technol ; 43(9): 3329-34, 2009 May 01.
Article en En | MEDLINE | ID: mdl-19534154
Large discharges from oil and gas production platforms (produced water) have led to concerns for adverse biological effects in marine areas. The aim of this study was to investigate the development of DNA adductformation and related biomarkers in fish after chronic exposure to water-soluble components of oil. Atlantic cod (Gadus morhua) were exposed for up to 44 weeks to three treatments (low, pulsed, high) containing environmentally relevant concentrations of low-molecular-weight polycyclic aromatic hydrocarbons (PAHs) and short-chained alkylphenols (APs). A time- and dose-related pattern of DNA adduct formation (measured using 32P-postlabeling) was observed. The results suggested that an extended exposure period (more than 16 weeks) would be required for the formation of DNA adduct levels above background. Interestingly, fish receiving pulsed high exposure did not develop elevated concentrations of DNA adducts, possibly due to DNA repair processes. No obvious relationship between DNA adduct concentration and cytochrome P4501A activity (EROD) was observed. This study has demonstrated the genotoxic potential of water-soluble oil components, relevant for operational discharges (produced water) and chronic oil spills. The quantification of PAH metabolites in bile and hepatic DNA adduct formation appear to be suitable for environmental monitoring of chronic oil pollution.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Contaminantes Químicos del Agua / Aceites / Gadus morhua / Ambiente Límite: Animals Idioma: En Revista: Environ Sci Technol Año: 2009 Tipo del documento: Article País de afiliación: Noruega Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Contaminantes Químicos del Agua / Aceites / Gadus morhua / Ambiente Límite: Animals Idioma: En Revista: Environ Sci Technol Año: 2009 Tipo del documento: Article País de afiliación: Noruega Pais de publicación: Estados Unidos