Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity.
Lab Chip
; 9(13): 1850-8, 2009 Jul 07.
Article
en En
| MEDLINE
| ID: mdl-19532959
We describe a highly efficient microfluidic fluorescence-activated droplet sorter (FADS) combining many of the advantages of microtitre-plate screening and traditional fluorescence-activated cell sorting (FACS). Single cells are compartmentalized in emulsion droplets, which can be sorted using dielectrophoresis in a fluorescence-activated manner (as in FACS) at rates up to 2000 droplets s(-1). To validate the system, mixtures of E. coli cells, expressing either the reporter enzyme beta-galactosidase or an inactive variant, were compartmentalized with a fluorogenic substrate and sorted at rates of approximately 300 droplets s(-1). The false positive error rate of the sorter at this throughput was <1 in 10(4) droplets. Analysis of the sorted cells revealed that the primary limit to enrichment was the co-encapsulation of E. coli cells, not sorting errors: a theoretical model based on the Poisson distribution accurately predicted the observed enrichment values using the starting cell density (cells per droplet) and the ratio of active to inactive cells. When the cells were encapsulated at low density ( approximately 1 cell for every 50 droplets), sorting was very efficient and all of the recovered cells were the active strain. In addition, single active droplets were sorted and cells were successfully recovered.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Beta-Galactosidasa
/
Microfluídica
/
Escherichia coli
/
Citometría de Flujo
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Lab Chip
Asunto de la revista:
BIOTECNOLOGIA
/
QUIMICA
Año:
2009
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Reino Unido