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Methylation of the sterol nucleus by STRM-1 regulates dauer larva formation in Caenorhabditis elegans.
Hannich, J Thomas; Entchev, Eugeni V; Mende, Fanny; Boytchev, Hristio; Martin, René; Zagoriy, Vyacheslav; Theumer, Gabriele; Riezman, Isabelle; Riezman, Howard; Knölker, Hans-Joachim; Kurzchalia, Teymuras V.
Afiliación
  • Hannich JT; Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany.
Dev Cell ; 16(6): 833-43, 2009 Jun.
Article en En | MEDLINE | ID: mdl-19531354
In response to pheromone(s), Caenorhabditis elegans interrupts its reproductive life cycle and enters diapause as a stress-resistant dauer larva. This decision is governed by a complex system of neuronal and hormonal regulation. All the signals converge onto the nuclear hormone receptor DAF-12. A sterol-derived hormone, dafachronic acid (DA), supports reproductive development by binding to DAF-12 and inhibiting its dauer-promoting activity. Here, we identify a methyltransferase, STRM-1, that modulates DA levels and thus dauer formation. By modifying the substrates that are used for the synthesis of DA, STRM-1 can reduce the amount of hormone produced. Loss of STRM-1 function leads to elevated levels of DA and inefficient dauer formation. Sterol methylation was not previously recognized as a mechanism for regulating hormone activity. Moreover, the C-4 sterol nucleus methylation catalyzed by STRM-1 is unique to nematodes and thus could be a target for therapeutic strategies against parasitic nematode infections.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Esteroles / Caenorhabditis elegans / Proteínas de Caenorhabditis elegans / Metiltransferasas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Dev Cell Asunto de la revista: EMBRIOLOGIA Año: 2009 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Esteroles / Caenorhabditis elegans / Proteínas de Caenorhabditis elegans / Metiltransferasas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Dev Cell Asunto de la revista: EMBRIOLOGIA Año: 2009 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos