Femtosecond optical transfection of cells: viability and efficiency.

Stevenson, D; Agate, B; Tsampoula, X; Fischer, P; Brown, C T A; Sibbett, W; Riches, A; Gunn-Moore, F; Dholakia, K

Stevenson, D; Agate, B; Tsampoula, X; Fischer, P; Brown, C T A; Sibbett, W; Riches, A; Gunn-Moore, F; Dholakia, K.

Afiliación

Stevenson D; Interdisciplinary Centre for Medical Photonics, School of Physics & Astronomy, University of St Andrews, St Andrews, KY16 9SS, Scotland, UK. ds50@st-andrews.ac.uk

Opt Express ; 14(16): 7125-33, 2006 Aug 07.

Article en En | MEDLINE | ID: mdl-19529083

RESUMEN

Photoporation is a rapidly expanding technique for the introduction of macromolecules into single cells. However, there remains no study into the true efficiency of this procedure. Here, we present a detailed analysis of transfection efficiency and cell viability for femtosecond optical transfection using a titanium sapphire laser at 800 nm. Photoporation of 4000 Chinese Hamster ovary cells was performed, representing the largest optical transfection study reported to date. We have investigated a range of laser fluences at the cell membrane and, at 1.2 microJ/cm(2), have found an average transfection efficiency of 50 +/- 10%. Contrary to recent literature, in which 100% efficiency is claimed, our measure of efficiency accounts for all irradiated cells, including those lost as a result of laser treatment, thereby providing a true biological measure of the technique.

Asunto(s)

Supervivencia Celular/fisiología; Ovario/citología; Transfección/métodos; Animales; Cricetinae; Femenino; Láseres de Semiconductores; Fenómenos Ópticos; Pinzas Ópticas

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ovario / Transfección / Supervivencia Celular Límite: Animals Idioma: En Revista: Opt Express Asunto de la revista: OFTALMOLOGIA Año: 2006 Tipo del documento: Article Pais de publicación: Estados Unidos

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