[SiRNA-mediated silencing of 5-lipoxygenase gene (ALOX5) reduces necrosis of neonatal cardiomyocytes in anoxia-reoxygenation].
Fiziol Zh (1994)
; 55(3): 37-43, 2009.
Article
en Uk
| MEDLINE
| ID: mdl-19526855
In experiments on the primary culture of isolated neonatal rat cardiomyocytes it was determined that cardiomyocytes express ALOX5 gene encoding enzyme 5-lipoxygenase. Anoxia-reoxygenation does not affect significantly the expression of 5-lipoxygenase mRNA in cardiomyocytes. Transfection of 5-lipoxygenase-specific small interfering RNA's (siRNA) into cardiomyocytes lead to a significant reduction of 5-lipoxygenase mRNA expression in cardiomyocytes 24 hours after transfection. ALOX5 gene silencing resulted in improved viability of cell population (by 13.3% P < 0.001) due to decreased number of necrotic (by 14.6%, P < 0.001), but not apoptotic, cells during anoxia-reoxygenation. Our results indicate that siRNA against ALOX5 effectively protects cardiomyocytes against anoxia-reoxygenation injury.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oxígeno
/
Araquidonato 5-Lipooxigenasa
/
Silenciador del Gen
/
Miocitos Cardíacos
/
ARN Interferente Pequeño
Límite:
Animals
Idioma:
Uk
Revista:
Fiziol Zh (1994)
Año:
2009
Tipo del documento:
Article
Pais de publicación:
Ucrania