A new mechanism of SOX9 action to regulate PKCalpha expression in the intestine epithelium.
J Cell Sci
; 122(Pt 13): 2191-6, 2009 Jul 01.
Article
en En
| MEDLINE
| ID: mdl-19509063
Variations of protein kinase C (PKC) expression greatly influence the proliferation-to-differentiation transition (PDT) of intestinal epithelial cells and might have an important impact on intestinal tumorigenesis. We demonstrate here that the expression of PKCalpha in proliferating intestinal epithelial cells is repressed both in vitro and in vivo by the SOX9 transcription factor. This repression does not require DNA binding of the SOX9 high-mobility group (HMG) domain but is mediated through a new mechanism of SOX9 action requiring the central and highly conserved region of SOXE members. Because SOX9 expression is itself upregulated by Wnt-APC signaling in intestinal epithelial cells, the present study points out this transcription factor as a molecular link between the Wnt-APC pathway and PKCalpha. These results provide a potential explanation for the decrease of PKCalpha expression in colorectal cancers with constitutive activation of the Wnt-APC pathway.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Regulación Enzimológica de la Expresión Génica
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Proteína Quinasa C-alfa
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Factor de Transcripción SOX9
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Mucosa Intestinal
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Cell Sci
Año:
2009
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Reino Unido