Isolation of T-lymphocytes from disaggregated tumors, with high purity and good percentage recovery.
J Natl Cancer Inst
; 59(1): 273-5, 1977 Jul.
Article
en En
| MEDLINE
| ID: mdl-195071
A combination of two cell separation methods was utilized for the isolation of thymus-derived lymphocytes (TL) from enzymatically disaggregated tumors. Passage through Sephadex G-10-glass bead columns to remove adherent cell types followed by exposure to IgG-coated sheep red blood cell monolayers for removal of Fc receptor-bearing inflammatory cells provided functional TL suspensions of high purity with good percentage recovery.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Linfocitos T
/
Separación Celular
/
Neoplasias Experimentales
Tipo de estudio:
Etiology_studies
Límite:
Animals
Idioma:
En
Revista:
J Natl Cancer Inst
Año:
1977
Tipo del documento:
Article
Pais de publicación:
Estados Unidos