Effects of n-acetylcysteine on ischemic preconditioning: study in isolated rat hearts.
Rev Bras Cir Cardiovasc
; 24(1): 23-30, 2009.
Article
en En, Pt
| MEDLINE
| ID: mdl-19504015
OBJECTIVE: The aim of this study is to assess if N-Acetylcysteine (NAC) changes the Ischemic Preconditioning (IP) in isolated rat hearts using only one cycle of IP. METHODS: Heart Rate (HR), Coronary Flow (CF) and Myocardial Contractility (dP/dt) were registered in 30 Wistar rat's hearts. After anesthesia the hearts were removed and perfused with Krebes-Hensleit equilibrated solution with 95% of O2 and 5% of CO2 according Langendorff's method. GI: Control (n=6); GII: 20 min. ischemia (n=6); GIII: IP (n=6); GIV 50 microg/ml/min NAC before IP (n =6); GV: 100 microg/ ml/min NAC before IP (n=6). Parameters were measured after 15 min. of stabilization (T 0) and T3, T5, T10, T15, T20, T25 and T30 min. after reperfusion. Statistical significance was considered when P<0.05. RESULTS: There were changes on HR comparing GI with GII at T20 and T25 and comparing GI with GIII, GIV with GV at T10 and T20 (P<0.05). CF was different comparing GI with GII at T3 and T5, GI with GIV at T10 and GI with GV at T10 and T25 (P<0.05). Myocardial Contractility was similar comparing GIII with GI and GV. GIII had higher dP/dt than GIV but without statistical difference (P>0.05). dP/dt was higher in GV than GIV but with statistically significant difference only at T30. CONCLUSION: dP/dt was better in preconditioned hearts and was changed if using NAC in GIV. The use of NAC didn't change the effects of preconditioning on myocardial contractility in GV.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Acetilcisteína
/
Depuradores de Radicales Libres
/
Precondicionamiento Isquémico Miocárdico
/
Circulación Coronaria
/
Frecuencia Cardíaca
/
Contracción Miocárdica
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
/
Pt
Revista:
Rev Bras Cir Cardiovasc
Asunto de la revista:
ANGIOLOGIA
/
CARDIOLOGIA
Año:
2009
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Brasil