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Effect of age, smoking and other lifestyle factors on urinary 7-methylguanine and 8-hydroxydeoxyguanosine.
Tamae, Kazuyoshi; Kawai, Kazuaki; Yamasaki, Sayumi; Kawanami, Kiyoshi; Ikeda, Masato; Takahashi, Ken; Miyamoto, Toshiaki; Kato, Noritada; Kasai, Hiroshi.
Afiliación
  • Tamae K; Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health.
Cancer Sci ; 100(4): 715-21, 2009 Apr.
Article en En | MEDLINE | ID: mdl-19469016
Urinary 8-hydroxydeoxyguanosine (8-OH-dG) and 7-methylguanine (m7Gua) were measured by a column-switching high performance liquid chromatography method as markers of oxidative and methylating DNA damage, respectively. We investigated the associations between urinary 8-OH-dG or m7Gua and various lifestyle and demographic factors, such as age and sex. The urinary 8-OH-dG excretion level was positively correlated with cigarette smoking, but inversely correlated with fruit consumption, physical activity and total energy consumed per day. A multiple regression analysis revealed that daily physical activity and healthy meal combinations decreased the urinary 8-OH-dG level, whereas alcohol consumption increased it. In terms of the urinary m7Gua measurement, cigarette smoking, age and consumption of meat, fish, egg, soybean, etc. were positively correlated with the urinary m7Gua level, whereas body weight, BMI, physical activity, and dietary index score, which indicates good nutritional balance, were negatively correlated with the amount of m7Gua. Based on a multiple regression analysis, cigarette smoking and age correlated with the m7Gua level, while high BMI and healthy meal combinations have significant reducing effects on m7Gua level. Therefore, the urinary m7Gua level is considered to be a useful marker of DNA methylation, not only from smoking, but also from aging and unhealthy dietary habits.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fumar / Desoxiguanosina / Conducta Alimentaria / Guanina / Estilo de Vida Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Adolescent / Adult / Humans / Male / Middle aged Idioma: En Revista: Cancer Sci Año: 2009 Tipo del documento: Article Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fumar / Desoxiguanosina / Conducta Alimentaria / Guanina / Estilo de Vida Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Adolescent / Adult / Humans / Male / Middle aged Idioma: En Revista: Cancer Sci Año: 2009 Tipo del documento: Article Pais de publicación: Reino Unido