Intracellular uptake of a new boronated porphyrin EC032.
Appl Radiat Isot
; 67(7-8 Suppl): S94-6, 2009 Jul.
Article
en En
| MEDLINE
| ID: mdl-19410469
We measured the toxicity and intracellular uptake of a newly developed boronated porphyrin EC032, and verified the fluorescence-based boron concentration measuring methods. Toxicity study showed that concentration required to produce a 50% reduction in viability (IC(50)) of EC032 was more than 0.25 mM. Fluorescence study showed the intracellular uptake of EC032 increased up until 24 h after its exposure to C6, 9L, U87, and U251 cells. There was also a linear correlation between ICP-AES and fluorescence intensity as an arbitrary unit about measurement of boron concentration. Fluorescence-based boron concentration measuring methods are very simple and useful methods, especially for screening of slight test dose of porphyrin compounds.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fármacos Sensibilizantes a Radiaciones
/
Compuestos de Boro
/
Terapia por Captura de Neutrón de Boro
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Appl Radiat Isot
Asunto de la revista:
MEDICINA NUCLEAR
/
SAUDE AMBIENTAL
Año:
2009
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Reino Unido