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SwissPIT: An workflow-based platform for analyzing tandem-MS spectra using the Grid.
Quandt, Andreas; Masselot, Alexandre; Hernandez, Patricia; Hernandez, Céline; Maffioletti, Sergio; Appel, Ron D; Lisacek, Frederique.
Afiliación
  • Quandt A; Swiss Institute of Bioinformatics, Proteome Informatics Group, Geneva, Switzerland. andreas.quandt@isb-sib.ch
Proteomics ; 9(10): 2648-55, 2009 May.
Article en En | MEDLINE | ID: mdl-19391179
The identification and characterization of peptides from MS/MS data represents a critical aspect of proteomics. It has been the subject of extensive research in bioinformatics resulting in the generation of a fair number of identification software tools. Most often, only one program with a specific and unvarying set of parameters is selected for identifying proteins. Hence, a significant proportion of the experimental spectra do not match the peptide sequences in the screened database due to inappropriate parameters or scoring schemes. The Swiss protein identification toolbox (swissPIT) project provides the scientific community with an expandable multitool platform for automated in-depth analysis of MS data also able to handle data from high-throughput experiments. With swissPIT many problems have been solved: The missing standards for input and output formats (A), creation of analysis workflows (B), unified result visualization (C), and simplicity of the user interface (D). Currently, swissPIT supports four different programs implementing two different search strategies to identify MS/MS spectra. Conceived to handle the calculation-intensive needs of each of the programs, swissPIT uses the distributed resources of a Swiss-wide computer Grid (http://www.swing-grid.ch).
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Programas Informáticos / Proteínas / Proteómica / Espectrometría de Masas en Tándem Tipo de estudio: Prognostic_studies Idioma: En Revista: Proteomics Asunto de la revista: BIOQUIMICA Año: 2009 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Alemania

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Programas Informáticos / Proteínas / Proteómica / Espectrometría de Masas en Tándem Tipo de estudio: Prognostic_studies Idioma: En Revista: Proteomics Asunto de la revista: BIOQUIMICA Año: 2009 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Alemania