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[Identification of Legionella spp. by means of cultural and molecular methods in a public hospital]. / Isolamento di Legionella spp. mediante metodi colturali e tecniche molecolari in matrici ambientali prelevate in una struttura ospedaliera pubblica.
Madeddu, M A; Carta, F; Muggianu, R; Meloni, P; Ingianni, A; Contu, A; Pompei, R.
Afiliación
  • Madeddu MA; Dipartimento di Scienze e Tecnologie Biomediche, Sezione di Microbiologia Applicata e Tecnologie Biomediche, Università degli Studi di Cagliari. mededduma@pacs.unica.it
Ann Ig ; 21(1): 71-7, 2009.
Article en It | MEDLINE | ID: mdl-19385336
An epidemiologic study on the isolation of Legionella spp from the sanitary water of a public Hospital in Cagliari (Italy) has been performed. The aim of the study was the comparison between the isolation of various Legionella spp from different hospital sources and the real hazard of Legionella infection of the inpatients. Two test methods were used for Legionella detection: a) the culture on selective media, that has the disadvantage of being quite time-consuming and of isolating also other bacterial species. Furthermore, the culture method often fails the isolation of vital but not culturable bacteria (VBNC); b) the PCR molecular method, which is rapid and precise and recognizes also VBNC cells. The most relevant result of this work was that, in spite of the isolation of a considerable number of Legionella spp (even Legionella pneumophila), no case of infection was detected in the Hospital during the period of the study.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Microbiología del Agua / Abastecimiento de Agua / Legionella / Hospitales Públicos Tipo de estudio: Diagnostic_studies / Prognostic_studies País/Región como asunto: Europa Idioma: It Revista: Ann Ig Asunto de la revista: MICROBIOLOGIA / SAUDE PUBLICA Año: 2009 Tipo del documento: Article Pais de publicación: Italia
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Microbiología del Agua / Abastecimiento de Agua / Legionella / Hospitales Públicos Tipo de estudio: Diagnostic_studies / Prognostic_studies País/Región como asunto: Europa Idioma: It Revista: Ann Ig Asunto de la revista: MICROBIOLOGIA / SAUDE PUBLICA Año: 2009 Tipo del documento: Article Pais de publicación: Italia