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Metformin increases insulin-stimulated glucose transport in insulin-resistant human skeletal muscle.
Galuska, D; Zierath, J; Thörne, A; Sonnenfeld, T; Wallberg-Henriksson, H.
Afiliación
  • Galuska D; Department of Clinical Physiology, Karolinska Hospital, Stockholm, Sweden.
Diabete Metab ; 17(1 Pt 2): 159-63, 1991 May.
Article en En | MEDLINE | ID: mdl-1936469
The effect of metformin (0.1 mM) on glucose transport was investigated in healthy control and in insulin-resistant human skeletal muscle. Muscle samples (200-400 mg) were obtained from the rectus abdominis muscle (abdominal surgery) or from the vastus lateralis portion of the quadriceps femoris muscle (open biopsy technique) from 8 healthy controls (age 38 +/- 4 yrs, BMI 23 +/- 1) and from 6 insulin-resistant subjects (age 53 +/- 5 yrs, BMI 30 +/- 2). Metformin had no effect on basal or insulin-stimulated (100 microU/ml) 3-0-methylglucose transport in incubated muscle strips from healthy subjects. Muscle tissue from the insulin resistant group did not respond to 100 microU/ml of insulin (0.73 +/- 0.17 for basal and 0.81 +/- 0.22 mumol x ml-1 x h-1 for insulin-stimulation, NS). Basal glucose transport was unaffected by metformin, whereas insulin-stimulated (100 microU/ml) glucose transport was increased by 63% in the insulin-resistant muscles (0.73 +/- 0.17 in the absence vs 1.19 +/- 0.18 mumol x ml-1 x h-1 in the presence of metformin, p less than 0.05). In conclusion, metformin abolishes insulin-resistance in human skeletal muscle by normalizing insulin-stimulated glucose transport accross the muscle cell membrane. The mechanism for this effect remains to be elucidated.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Resistencia a la Insulina / Glucosa / Insulina / Metformina / Músculos Límite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Diabete Metab Año: 1991 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Francia
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Resistencia a la Insulina / Glucosa / Insulina / Metformina / Músculos Límite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Diabete Metab Año: 1991 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Francia