Flow cytometric analysis of T-lymphocytes from nasopharynx-associated lymphoid tissue (NALT) in a model of secondary immunodeficiency in Wistar rats.
Immunobiology
; 214(5): 384-91, 2009.
Article
en En
| MEDLINE
| ID: mdl-19362684
Nasopharynx-associated lymphoid tissue (NALT) is responsible for immune responses in the upper respiratory tract of rodents. In our model of protein malnutrition (R21 group), bronchus-associated lymphoid tissue (BALT), situated in the lower respiratory tract, showed a decrease of CD4(+), CD8alpha(+), and TCRalphabeta(+) lymphocytes but TCRgammadelta(+) cells were increased. Besides, there is no information regarding the frequencies of T-cell populations in 60-day-old Wistar rats (C60 group). So, the aim of the present study was to analyze by flow cytometry NALT T-cells from both groups. NALT lymphocytes were isolated from R21 and C60 groups and stained with different antibodies. Samples were run on a FACScalibur flow cytometer. Background staining was evaluated using isotype controls. Data analysis was performed using BD Cell Quest and WinMDI 2.9. In C60, the predominant population was CD4(+)TCRalphabeta(+), which was significantly diminished in the R21 group. However, CD8alpha(+), the majority expressing CD8alphabeta, and TCRgammadelta(+) cells were not affected. In our model of secondary immunodeficiency, there is a compartmentalization between NALT and BALT because they differ in the populations affected even though they are inductive sites of the respiratory tract in the common mucosal immune system.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Nasofaringe
/
Subgrupos de Linfocitos T
/
Desnutrición Proteico-Calórica
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Síndromes de Inmunodeficiencia
/
Tejido Linfoide
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
Idioma:
En
Revista:
Immunobiology
Año:
2009
Tipo del documento:
Article
País de afiliación:
Argentina
Pais de publicación:
Países Bajos