Expression of functional metallothionein isoforms in papillary thyroid cancer.
Mol Cell Endocrinol
; 302(1): 92-8, 2009 Apr 10.
Article
en En
| MEDLINE
| ID: mdl-19356627
Metallothionein (MT) isoforms have not been studied in papillary thyroid cancer. We examined how the functional MT1 and MT2 isoforms were expressed in papillary thyroid cancer (KAT5) cells. We demonstrated that KAT5 cells expressed eight functional MT1 and MT2 isoforms induced by cadmium. Elevated calcium and activated ERK1/2 predated MT expression. The inhibition of either calcium or ERK1/2 significantly blocked the isoform expression. The induction of these isoforms accompanied an increased progression of cell cycle from G0/G1 to G2-M. The alternation in cell cycle disappeared when the expression of MT isoforms was blocked by calcium inhibitor or ERK1/2 inhibitor. Collectively, KAT5 cells express eight functional MT1 and MT2 isoforms in a pathway controlled by calcium and ERK1/2. The elevation of the MT isoforms contributes to the decreased G0/G1 but increased G2-M phase. These results reveal a novel pathway for the expression of the functional MT in papillary thyroid cancer.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias de la Tiroides
/
Carcinoma Papilar
/
Regulación Neoplásica de la Expresión Génica
/
Isoformas de Proteínas
/
Metalotioneína
Límite:
Humans
Idioma:
En
Revista:
Mol Cell Endocrinol
Año:
2009
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Irlanda