Optical calibration protocol for an x-ray and optical multimodality tomography system dedicated to small-animal examination.
Appl Opt
; 48(10): D151-62, 2009 Apr 01.
Article
en En
| MEDLINE
| ID: mdl-19340104
A small-animal multimodality tomography system dedicated to the coregistration of fluorescence optical signal and x-ray measurements has been developed in our laboratory. The purpose of such a system is to offer the possibility of getting in vivo anatomical and functional information simultaneously. Moreover, anatomical measurements can be used as a regularization factor to achieve more accurate reconstructions of the biodistribution of fluorochromes and to speed up treatment. A dedicated acquisition protocol has been established, and the methodology of the reconstruction of the three-dimensional distribution of the biomarkers under cylindrical geometry consistent with classic computed tomography has been implemented. A phantom study was conducted to evaluate and to fix the parameters for the coregistration. These test experiments were reproduced by considering anesthetized mice that had thin glass tubes containing fluorochromes inserted into their esophagus. The instrument is also used for an in vivo biological study conducted on mice with lung tumors, tagged with near-infrared optical probes (targeting probes such as Transferin-AlexaFluor750).
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Tomografía Computarizada por Rayos X
/
Tomografía Óptica
/
Dispositivos Ópticos
Tipo de estudio:
Diagnostic_studies
/
Guideline
/
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Appl Opt
Año:
2009
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Estados Unidos