Non-isothermal kinetics of hard alpha-keratin thermal denaturation.
Macromol Biosci
; 9(8): 805-12, 2009 Aug 11.
Article
en En
| MEDLINE
| ID: mdl-19291668
DSC measurements carried out at different heating rates were used for the kinetic analysis of the endothermic process assigned to the denaturation of the helical material from human hair in water excess. We found that the kinetic mechanism is autocatalytic and that the value of the activation energy is close to disulphide bond scission rather than to protein denaturation. This allowed us to propose a multistep mechanism for the thermal denaturation of hard alpha-keratins in water excess that relies on the 3-phase model which describes their structure. The limiting step of the thermal denaturation process is then the scission of S-S bonds between the main morphological components, namely IF and matrix (IFAP). The theoretical proposed model shows a good agreement with the experimental recorded data.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Desnaturalización Proteica
/
Termodinámica
/
Queratinas
Idioma:
En
Revista:
Macromol Biosci
Asunto de la revista:
BIOQUIMICA
Año:
2009
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
Alemania