[Viability of corneal epithelial cells and cleavage of corneal basement after ethanol effect].
Zhonghua Yan Ke Za Zhi
; 44(12): 1088-92, 2008 Dec.
Article
en Zh
| MEDLINE
| ID: mdl-19187662
OBJECTIVE: To evaluate the viability of corneal epithelial cells and to determine the anatomic cleavage on the epithelial basement membrane after various exposure times to 20% ethanol during epithelial flap preparation in laser-assisted subepithelial keratectomy (LASEK) in cadaver eyes. METHODS: Six human cadaver eyes were exposed to 20% ethanol for 20, 30 and 40 seconds (2 eyes for each group), and another one eye was used as the control. PCNA staining was performed to determine the viability of corneal epithelial cells. Immunofluorescence staining using monoclonal antibodies against collagen VII, and immunohistological staining using monoclonal antibodies against laminin were performed to detect the anatomic location of the cleavage plane on the corneal epithelial flaps created by 20 seconds exposure to 20% ethanol in cadaver eyes. RESULTS: Hematoxylin and eosin staining of epithelial flaps revealed a coherent stratified epithelium. The PCNA positive rates of the epithelial cells in the flap decreased in the 20-second group, 30-second group and 40-second group successively. Immunohistological staining to laminin was patchy in the lifted flap and the remaining corneal basement membrane. Immunofluorescence to collagen VII, the main component of anchoring fibrils remained exclusively in the corneal bed. CONCLUSIONS: Viability of the epithelial flap decreased with longer time exposure to ethanol. The cleavage plane of the ethanol-treated corneal epithelial flap is located between the lamina lucida and the lamina densa of the basement membrane where laminin forms hemidesmosome.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Colgajos Quirúrgicos
/
Membrana Basal
/
Epitelio Corneal
/
Etanol
Límite:
Humans
Idioma:
Zh
Revista:
Zhonghua Yan Ke Za Zhi
Año:
2008
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
China