On the mechanism of oligomycin inhibition of Ca(2+)-induced mitochondrial respiration.

Krasinskaya, I P; Kudryashova, I A; Yaguzhinsky, L S

Krasinskaya, I P; Kudryashova, I A; Yaguzhinsky, L S.

Afiliación

Krasinskaya IP; A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, USSR.

FEBS Lett ; 290(1-2): 52-4, 1991 Sep 23.

Article en En | MEDLINE | ID: mdl-1915891

RESUMEN

The addition of oligomycin in the presence of Ca2+ increased the ADP pool in mitochondrial suspension. It is suggested that oligomycin inhibition of Ca(2+)-induced mitochondrial respiratory activation is the function of the increased endogenous ADP pool. Low ADP concentrations (5-20 microM) produce the same inhibitory effect as oligomycin. The increase of ADP levels in the presence of glucose plus hexokinase resulted in the inhibition of Ca(2+)-induced respiration, while the addition of phosphoenol pyruvate plus pyruvate kinase followed by a reduction in ADP levels, reversed the oligomycin inhibitory effect. One of the essential stages of ADP accumulation in mitochondrial suspensions in the presence of oligomycin and Ca2+ is proposed to be the formation of ADP from AMP and ATP, effected by adenylate kinase.

Asunto(s)

Nucleótidos de Adenina/metabolismo; Calcio/farmacología; Mitocondrias Hepáticas/efectos de los fármacos; Oligomicinas/farmacología; Consumo de Oxígeno/efectos de los fármacos; Adenilato Quinasa/metabolismo; Animales; Atractilósido/análogos & derivados; Atractilósido/farmacología; Técnicas In Vitro; Mitocondrias Hepáticas/metabolismo; Translocasas Mitocondriales de ADP y ATP/metabolismo; Ratas

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oligomicinas / Consumo de Oxígeno / Mitocondrias Hepáticas / Nucleótidos de Adenina / Calcio Límite: Animals Idioma: En Revista: FEBS Lett Año: 1991 Tipo del documento: Article Pais de publicación: Reino Unido

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