MR molecular imaging of thrombus: development and application of a Gd-based novel contrast agent targeting to P-selectin.
Clin Appl Thromb Hemost
; 16(2): 177-83, 2010 Apr.
Article
en En
| MEDLINE
| ID: mdl-19141485
Molecular imaging of thrombus formation at initial stage requires a robust thrombus-specific contrast agent with high sensitivity. In this study, we report a novel P-selectin-targeted paramagnetic molecular imaging agent and the agent's potential to sensitively detect occult microthrombi on the intimal surface of endothelium. Platelet clots and blood clots targeted in vitro with paramagnetic nanoparticles presented a highly detectable, homogeneous T1-weighted contrast enhancement that was improved with increasing gadolinium level. In vivo contrast enhancement under part of circulation conditions was assessed in dogs. The micro-thrombi around the femoral vein of dog demonstrated higher signal intensities than the control clots and the adjacent muscle. Histology was performed on regions likely to contain thrombus as indicated by MRI. These results suggest that molecular imaging of P-selectin-targeted paramagnetic nanoparticles can provide sensitive detection and localization of P-selectin and may allow for early, direct identification of microthrombi, leading to early diagnosis.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Albúmina Sérica Bovina
/
Imagen por Resonancia Magnética
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Inmunoconjugados
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Selectina-P
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Medios de Contraste
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Gadolinio DTPA
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Trombosis de la Vena
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Imagenología Tridimensional
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Nanopartículas
/
Imagen Molecular
Tipo de estudio:
Screening_studies
Límite:
Animals
Idioma:
En
Revista:
Clin Appl Thromb Hemost
Asunto de la revista:
ANGIOLOGIA
Año:
2010
Tipo del documento:
Article
Pais de publicación:
Estados Unidos