beta-glucosidase catalyzed syntheses of pyridoxine glycosides.

Charles, Rajachristu Einstein; Divakar, Soundar

Charles, Rajachristu Einstein; Divakar, Soundar.

Afiliación

Charles RE; Fermentation Technology and Bioengineering Department, Central Food Technological Research Institute, Council of Scientific and Industrial Research, Mysore, India.

Biosci Biotechnol Biochem ; 73(1): 233-6, 2009 Jan.

Article en En | MEDLINE | ID: mdl-19129657

RESUMEN

Enzymatic syntheses of pyridoxine glycosides were carried out in di-isopropyl ether organic medium using beta-glucosidase isolated from sweet almond. Optimum conditions determined for the reaction with D-glucose were 40% (w/w D-glucose) beta-glucosidase at 0.18 mM (1.8 ml) of pH 5 acetate buffer over a 72 h incubation period. Of 11 carbohydrates employed, beta-glucosidase gave 7-O-(alpha-D-glucopyranosyl)pyridoxine 5a, 7-O-(beta-D-glucopyranosyl)pyridoxine 5b, 6-O-(alpha-D-glucopyranosyl)pyridoxine 5c, 7-O-(alpha-D-galactopyranosyl)pyridoxine 6a, 7-O-(beta-D-galactopyranosyl)pyridoxine 6b, 6-O-(alpha-D-galactopyranosyl)pyridoxine 6c, 7-O-(alpha-D-mannopyranosyl)pyridoxine 7a, 7-O-(beta-D-mannopyranosyl)pyridoxine 7b, and 6-O-(alpha-D-mannopyranosyl)pyridoxine 7c in yields ranging from 23 to 40%.

Asunto(s)

Glicósidos/síntesis química; Piridoxina/síntesis química; beta-Glucosidasa/metabolismo; Carbohidratos; Glicósidos/análisis; Prunus/enzimología; Piridoxina/análisis; Solventes

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Piridoxina / Beta-Glucosidasa / Glicósidos Idioma: En Revista: Biosci Biotechnol Biochem Asunto de la revista: BIOQUIMICA / BIOTECNOLOGIA Año: 2009 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido

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