User configurable microfluidic device for multiplexed immunoassays based on DNA-directed assembly.

Schroeder, Hendrik; Adler, Michael; Gerigk, Katrin; Müller-Chorus, Birgit; Götz, Friedrich; Niemeyer, Christof M

Schroeder, Hendrik; Adler, Michael; Gerigk, Katrin; Müller-Chorus, Birgit; Götz, Friedrich; Niemeyer, Christof M.

Afiliación

Schroeder H; Technische Universität Dortmund, Fakultät Chemie, Biologisch-Chemische Mikrostrukturtechnik, Otto Hahn Str. 6, 44227 Dortmund, Germany.

Anal Chem ; 81(3): 1275-9, 2009 Feb 01.

Article en En | MEDLINE | ID: mdl-19123774

RESUMEN

We present a microfluidic device for multiplexed immunoassays based on DNA-directed immobilization. Because of the versatile building blocks used for this technique, it is possible to build up user configurable protein microarrays within the microfluidic system by means of DNA-directed self-assembly, which can be used for immunoassay applications. We demonstrate the performance of our system by parallel detection of cytokines in a multiplex immunoassay, employing silver deposition labeling and optical read-out.

Asunto(s)

ADN/química; Inmunoensayo/métodos; Técnicas Analíticas Microfluídicas/métodos; Biomarcadores/análisis; Biomarcadores de Tumor/análisis; Análisis por Matrices de Proteínas

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Inmunoensayo / Técnicas Analíticas Microfluídicas Idioma: En Revista: Anal Chem Año: 2009 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos

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