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Intraventricular orexin-A improves arousal and early EEG entropy in rats after cardiac arrest.
Koenig, Matthew A; Jia, Xiaofeng; Kang, Xiaoxu; Velasquez, Adrian; Thakor, Nitish V; Geocadin, Romergryko G.
Afiliación
  • Koenig MA; The Johns Hopkins University School of Medicine, 600 N. Wolfe St, Meyer 8-140, Baltimore, MD 21287, USA. mkoenin1@jhmi.edu
Brain Res ; 1255: 153-61, 2009 Feb 19.
Article en En | MEDLINE | ID: mdl-19111527
The recovery of arousal after cardiac arrest (CA) is associated with evolution from electroencephalographic (EEG) burst-suppression to continuous activity. Orexin-A elicits arousal EEG during anesthetic burst-suppression. We hypothesized that orexin-A would improve arousal and EEG entropy after CA. Eighteen Wistar rats were subjected to 7-minute asphyxial CA and resuscitation. Rats were divided into treatment (n=9) and control (n=9) groups. Twenty minutes after resuscitation, the treatment group received 0.1 mL of 1 nM orexin-A intraventricularly, while controls received saline. EEG was quantified using Information Quantity (IQ), a measure of entropy validated for detection of burst-suppression and arousal patterns. IQ values range from 0 to 1.0. Arousal was quantified using the neurological deficit scale (NDS). The ischemic neuronal fraction of hippocampus CA1 and cortex was histologically determined. Baseline and post-resuscitation characteristics were similar between the groups. The NDS score (mean+/-SD) at 4 h was higher in the orexin-A group compared to controls (57.3+/-5.8 vs. 40.7+/-5.9, p<0.02), but scores were similar at 72 h. Burst frequency was similar in both groups but the orexin-A group demonstrated higher IQ values compared to controls beginning within 10 min. IQ values remained significantly higher in the orexin-A group for the first 120 min (p=0.008) and subsequently converged. The ischemic neuronal fraction was similar between groups in cortex (p=0.54) and hippocampus CA1 (p=0.14). In rats resuscitated from CA, orexin-A transiently increased arousal and EEG entropy without worsening ischemic neuronal injury. The role of orexin-A in recovery of arousal after CA deserves further investigation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nivel de Alerta / Neuropéptidos / Entropía / Péptidos y Proteínas de Señalización Intracelular / Electroencefalografía / Paro Cardíaco / Neuronas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Brain Res Año: 2009 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nivel de Alerta / Neuropéptidos / Entropía / Péptidos y Proteínas de Señalización Intracelular / Electroencefalografía / Paro Cardíaco / Neuronas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Brain Res Año: 2009 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos