Agrobacterium tumefaciens iron superoxide dismutases have protective roles against singlet oxygen toxicity generated from illuminated Rose Bengal.
FEMS Microbiol Lett
; 289(1): 97-103, 2008 Dec.
Article
en En
| MEDLINE
| ID: mdl-19054099
Singlet oxygen is a highly reactive form of molecular oxygen that is harmful to biological systems. Here, the role of three iron-containing superoxide dismutase (sodB) genes is clearly shown in protecting Agrobacterium tumefaciens against singlet oxygen toxicity. A sodBI mutant was more sensitive to singlet oxygen than both wild-type bacteria and a double sodBII-sodBIII mutant strain. Moreover, a sodBI-sodBII double mutant had higher sensitivity to singlet oxygen than a single sodBI mutant, although the double mutant was comparable to a sodB null mutant. High-level expression of sodBI and sodBII fully complemented the singlet oxygen hypersensitivity phenotype of the sodB null mutant, while high-level expression of sodBIII encoding a periplasmic SOD only partially restored the phenotype. Taken together, our data suggest that SodBI and SodBII have novel protective roles against singlet oxygen toxicity through unknown mechanisms.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Rosa Bengala
/
Superóxido Dismutasa
/
Regulación Bacteriana de la Expresión Génica
/
Agrobacterium tumefaciens
/
Oxígeno Singlete
/
Luz
Idioma:
En
Revista:
FEMS Microbiol Lett
Año:
2008
Tipo del documento:
Article
País de afiliación:
Tailandia
Pais de publicación:
Reino Unido