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In vivo characterization of transplanted human embryonic stem cell-derived pancreatic endocrine islet cells.
Eshpeter, A; Jiang, J; Au, M; Rajotte, R V; Lu, K; Lebkowski, J S; Majumdar, A S; Korbutt, G S.
Afiliación
  • Eshpeter A; Alberta Diabetes Institute andDepartment of Surgery, University of Alberta, Edmonton, Canada, andGeron Corporation, Menlo Park, CA, USA.
  • Jiang J; Alberta Diabetes Institute andDepartment of Surgery, University of Alberta, Edmonton, Canada, andGeron Corporation, Menlo Park, CA, USA.
  • Au M; Alberta Diabetes Institute andDepartment of Surgery, University of Alberta, Edmonton, Canada, andGeron Corporation, Menlo Park, CA, USA.
  • Rajotte RV; Alberta Diabetes Institute andDepartment of Surgery, University of Alberta, Edmonton, Canada, andGeron Corporation, Menlo Park, CA, USA.
  • Lu K; Alberta Diabetes Institute andDepartment of Surgery, University of Alberta, Edmonton, Canada, andGeron Corporation, Menlo Park, CA, USA.
  • Lebkowski JS; Alberta Diabetes Institute andDepartment of Surgery, University of Alberta, Edmonton, Canada, andGeron Corporation, Menlo Park, CA, USA.
  • Majumdar AS; Alberta Diabetes Institute andDepartment of Surgery, University of Alberta, Edmonton, Canada, andGeron Corporation, Menlo Park, CA, USA.
  • Korbutt GS; Alberta Diabetes Institute andDepartment of Surgery, University of Alberta, Edmonton, Canada, andGeron Corporation, Menlo Park, CA, USA.
Cell Prolif ; 41(6): 843-858, 2008 Dec.
Article en En | MEDLINE | ID: mdl-19040565
OBJECTIVES: Islet-like clusters (ILCs), differentiated from human embryonic stem cells (hESCs), were characterized both before and after transplantation under the kidney capsule of streptozotocin-induced diabetic immuno-incompetent mice. MATERIALS AND METHODS: Multiple independent ILC preparations (n = 8) were characterized by immunohistochemistry, flow cytometry and cell insulin content, with six preparations transplanted into diabetic mice (n = 42), compared to controls, which were transplanted with either a human fibroblast cell line or undifferentiated hESCs (n = 28). RESULTS: Prior to transplantation, ILCs were immunoreactive for the islet hormones insulin, C-peptide and glucagon, and for the ductal epithelial marker cytokeratin-19. ILCs also had cellular insulin contents similar to or higher than human foetal islets. Expression of islet and pancreas-specific cell markers was maintained for 70 days post-transplantation. The mean survival of recipients was increased by transplanted ILCs as compared to transplanted human fibroblast cells (P < 0.0001), or undifferentiated hESCs (P < 0.042). Graft function was confirmed by secretion of human C-peptide in response to an oral bolus of glucose. CONCLUSIONS: hESC-derived ILC grafts continued to contain cells that were positive for islet endocrine hormones and were shown to be functional by their ability to secrete human C-peptide. Further enrichment and maturation of ILCs could lead to generation of a sufficient source of insulin-producing cells for transplantation into patients with type 1 diabetes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trasplante de Islotes Pancreáticos / Islotes Pancreáticos / Células Madre Embrionarias / Células Endocrinas Límite: Animals / Humans Idioma: En Revista: Cell Prolif Año: 2008 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trasplante de Islotes Pancreáticos / Islotes Pancreáticos / Células Madre Embrionarias / Células Endocrinas Límite: Animals / Humans Idioma: En Revista: Cell Prolif Año: 2008 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido