Few alterations in clinical pathology and histopathology observed in a CYP2C18&19 humanized mice model.
Acta Vet Scand
; 50: 47, 2008 Nov 27.
Article
en En
| MEDLINE
| ID: mdl-19038035
BACKGROUND: This study was performed to characterize a gene-addition transgenic mouse containing a BAC (bacterial artificial chromosome) clone spanning the human CYP2C18&19 genes (tg-CYP2C18&19). METHODS: Hemizygous tg-CYP2C18&19, 11 week old mice were compared with wild-type littermates to obtain information regarding clinical status, clinical pathology and anatomical pathology. After one week of clinical observations, blood samples were collected, organs weighed, and tissues collected for histopathology. RESULTS: In males, the tissue weights were lower in tg-CYP2C18&19 than in wild-type mice for brain (p < or = 0.05), adrenal glands (p < or = 0.05) and brown fat deposits (p < or = 0.001) while the heart weight was higher (p < or = 0.001). In female tg-CYP2C18&19, the tissue weights were lower for brain (p < or = 0.001) and spleen (p < or = 0.001) compared to wild-type females. Male tg-CYP2C18&19 had increased blood glucose levels (p < or = 0.01) while females had decreased blood triglyceride levels (p < or = 0.01). CONCLUSION: Despite the observed alterations, tg-CYP2C18&19 did not show any macroscopic or microscopic pathology at the examined age. Hence, these hemizygous transgenic mice were considered to be viable and healthy animals.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Hidrocarburo de Aril Hidroxilasas
Límite:
Animals
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Female
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Humans
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Male
Idioma:
En
Revista:
Acta Vet Scand
Año:
2008
Tipo del documento:
Article
País de afiliación:
Suecia
Pais de publicación:
Reino Unido