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HSP70 expression in human trophoblast cells exposed to different 1.8 Ghz mobile phone signals.
Franzellitti, Silvia; Valbonesi, Paola; Contin, Andrea; Biondi, Carla; Fabbri, Elena.
Afiliación
  • Franzellitti S; Interdepartmental Centre for Environmental Science Research, University of Bologna, 48100 Ravenna, Italy.
Radiat Res ; 170(4): 488-97, 2008 Oct.
Article en En | MEDLINE | ID: mdl-19024656
The heat-shock proteins (HSPs) are important cellular stress markers and have been proposed as candidates to infer biological effects of high-frequency electromagnetic fields (EMFs). In the current study, HSP70 gene and protein expression were evaluated in cells of the human trophoblast cell line HTR-8/SVneo after prolonged exposure (4 to 24 h) to 1.8 GHz continuous-wave (CW) and different GSM signals (GSM-217Hz and GSM-Talk) to assess the possible effects of time and modulation schemes on cell responses. Inducible HSP70 protein expression was not modified by high-frequency EMFs under any condition tested. The inducible HSP70A, HSP70B and the constitutive HSC70 transcripts did not change in cells exposed to high-frequency EMFs with the different modulation schemes. Instead, levels of the inducible HSP70C transcript were significantly enhanced after 24 h exposure to GSM-217Hz signals and reduced after 4 and 16 h exposure to GSM-Talk signals. As in other cell systems, in HTR-8/SVneo cells the response to high-frequency EMFs was detected at the mRNA level after exposure to amplitude-modulated GSM signals. The present results suggest that the expression analysis for multiple transcripts, though encoding the same or similar protein products, can be highly informative and may account for subtle changes not detected at the protein level.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ondas de Radio / Trofoblastos / Proteínas HSP70 de Choque Térmico / Teléfono Celular Límite: Humans Idioma: En Revista: Radiat Res Año: 2008 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ondas de Radio / Trofoblastos / Proteínas HSP70 de Choque Térmico / Teléfono Celular Límite: Humans Idioma: En Revista: Radiat Res Año: 2008 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos