The influence of L-NG-nitro-arginine on field stimulation induced contractions and acetylcholine release in guinea pig isolated tracheal smooth muscle.
Biochem Biophys Res Commun
; 179(2): 1017-22, 1991 Sep 16.
Article
en En
| MEDLINE
| ID: mdl-1898382
The interaction between parasympathetic and inhibitory non-adrenergic, non-cholinergic nerves in tracheal smooth muscle was investigated by determining the effects of the NO-synthase inhibitor L-NG-nitro-arginine (L-NOARG) on contractions and the associated acetylcholine release elicited by field stimulation of the muscle. At frequencies above 2Hz contractile responses to field stimulation were potentiated by L-NOARG (50 microM). alpha-chymotrypsin pre-treatment potentiated contractile responses at all frequencies, but the effects of L-NOARG were unaltered. The effect of L-NOARG on responses to 5Hz electrical stimulation was not mimicked by D-NOARG, was reversed by L-, but not D-arginine and was unaffected by epithelium removal. L-NOARG did not affect responses to exogenous acetylcholine nor the overflow of 3H from tissues previously loaded with [3H]-choline. It is therefore concluded that field stimulation of tracheal smooth muscle induces the release of an endogenous nitrate, which, by an inhibitory action on smooth muscle, functionally antagonises the concomitantly released parasympathetic neurotransmitter.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Arginina
/
Acetilcolina
/
Contracción Muscular
/
Músculo Liso
Límite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
1991
Tipo del documento:
Article
Pais de publicación:
Estados Unidos