Normal saline induces oxidative stress in peritoneal mesothelial cells.
J Pediatr Surg
; 43(10): 1821-6, 2008 Oct.
Article
en En
| MEDLINE
| ID: mdl-18926214
BACKGROUND: Peritoneal adhesions are the most common complication of the abdominal surgery. Normal saline is frequently used to rinse the peritoneal cavity during abdominal surgery, although there is no well-established data describing effect of such procedure on the process of formation of peritoneal adhesions. METHODS: Effect of 0.9% NaCl solution on viability, oxidative stress, and fibrinolytic activity of human peritoneal mesothelial cells maintained in in vitro culture was evaluated. RESULTS: Exposure of mesothelial cells to 0.9% NaCl induces oxidative stress, derangement of their structure with subsequent increased release of tissue factor (+75%) and plasminogen activator inhibitor-1 (+19%), and simultaneous suppression of tissue plasminogen activator release (-39%). In effect, ration tissue plasminogen activator/plasminogen activator inhibitor-1 was reduced in 0.9% NaCl-treated cells by 50%. Pretreatment of cells with precursor of glutathione synthesis: L-2-oxothiazolidine-4-carboxylic acid prevented these changes. CONCLUSIONS: Oxidative stress in the peritoneal mesothelium caused by 0.9% NaCl activates their procoagulant activity and impairs fibrinolytic properties of these cells. These effects disqualify 0.9% NaCl as rinsing solution during abdominal surgery.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Epiplón
/
Cloruro de Sodio
/
Estrés Oxidativo
/
Células Epiteliales
Límite:
Humans
Idioma:
En
Revista:
J Pediatr Surg
Año:
2008
Tipo del documento:
Article
País de afiliación:
Polonia
Pais de publicación:
Estados Unidos