Trypan blue as a fluorochrome for confocal laser scanning microscopy of arbuscular mycorrhizae in three mangroves.
Biotech Histochem
; 83(3-4): 153-9, 2008 Jun.
Article
en En
| MEDLINE
| ID: mdl-18846451
Roots of three mangroves, Acanthus ilicifolius, Ceriops tagal and Excoecaria agallocha, collected from forests of the Sundarbans of India were stained with trypan blue to observe arbuscular mycorrhizal colonization. Spores of arbuscular mycorrhizal fungi isolated from rhizospheric soil, collected together with the root samples, also were stained for testing the suitability of the dye as a fluorochrome. Confocal laser scanning microscopy images were constructed. A. ilicifolius and E. agallocha exhibited "Arum" type colonization with highly branched arbuscules, whereas C. tagal showed "Paris" type association with clumped and collapsed arbuscules. We demonstrated that trypan blue is a suitable fluorochrome for staining arbuscular mycorrhizal fungal spores, fungal hyphae, arbuscules and vesicles, which presumably have a considerable amount of surface chitin. It appears that as the integration of chitin into the fungal cell wall changes, its accessibility to trypan blue dye also changes.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Azul de Tripano
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Raíces de Plantas
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Magnoliopsida
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Micorrizas
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Colorantes Fluorescentes
Idioma:
En
Revista:
Biotech Histochem
Asunto de la revista:
HISTOCITOQUIMICA
Año:
2008
Tipo del documento:
Article
País de afiliación:
India
Pais de publicación:
Reino Unido