Phenotype and mRNA expression of syncytiotrophoblast microparticles isolated from human placenta.
Ann N Y Acad Sci
; 1137: 144-7, 2008 Aug.
Article
en En
| MEDLINE
| ID: mdl-18837939
Fetal and neonatal alloimmune thromboctyopenia due to maternal human platelet antigen (HPA)-1a antibodies affects primigravidas. Immunization must occur early in pregnancy before fetal platelets enter maternal blood via fetomaternal hemorrhage. The HPA-1a antigen is located on platelet glycoprotein (GP)IIIa (CD61, beta3 integrin), which is also present on the placental syncytiotrophoblast (ST) and in direct contact with maternal blood. Since ST debris is shed into maternal blood during pregnancy, this material might be immunogenic in vivo. For experimental purposes, we prepared and characterized ST microparticles (STMPs) in vitro from term placentas. Phenotype analysis by flow cytometry and Western blotting showed that STMP expressed more placental alkaline phosphatase (PLAP) than GPIIIa. Quantitative real-time PCR demonstrated expression of human placental lactogen (HPL), human chorionic gonadotrophin (HCG), and GPIIIa by STMP, in the order HPL > HCG > GPIIIa. PLAP, HPL, and HCG are trophoblast-specific proteins. These STMPs may be a useful model for studying the natural ST debris in plasma of pregnant women.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fenotipo
/
Placenta
/
Trofoblastos
/
ARN Mensajero
Límite:
Female
/
Humans
/
Pregnancy
Idioma:
En
Revista:
Ann N Y Acad Sci
Año:
2008
Tipo del documento:
Article
País de afiliación:
Reino Unido
Pais de publicación:
Estados Unidos