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Correlation between high field MR images and histopathological findings of rat transplanted cancer immediately after partial microwave coagulation.
Murakami, Koichiro; Morikawa, Shigehiro; Naka, Shigeyuki; Demura, Koichi; Sato, Koichiro; Shiomi, Hisanori; Kurumi, Yoshimasa; Inubushi, Toshiro; Tani, Tohru.
Afiliación
  • Murakami K; Department of Surgery, Shiga University of Medical Science, Shiga, Japan. emkami@belle.shiga-med.ac.jp
Magn Reson Med Sci ; 7(3): 105-12, 2008.
Article en En | MEDLINE | ID: mdl-18827453
PURPOSE: To investigate the immediate effects of microwave coagulation on rat tumors in various magnetic resonance (MR) images at high magnetic field strength using histopathological examinations as reference. MATERIALS AND METHODS: Tumors implanted in rat femurs were partially thermocoagulated by microwave. Immediately after, T1- and T2-weighted images, diffusion-weighted images (DWIs), and contrast-enhanced T1 weighted images (CE-T1WIs) were acquired with a 7-tesla MR scanner. After measurements, tumors were examined histopathologically with hematoxylin-eosin (HE) staining and histochemically for acid phosphatase activity. RESULTS: Without contrast, boundaries of coagulated areas were unclear on MR images, including apparent diffusion coefficient (ADC) maps. CE-T1WIs clearly showed immediate contrast enhancement of untreated areas of tumor, and the area of enhancement gradually enlarged in 5 min. Quantitative analyses were conducted by classifying tumor areas by contrast enhancement results. Signal intensities of the areas in the MR images showed no significant differences, but at the periphery, ADC values were significantly higher in areas with delayed enhancement than those with immediate enhancement. Compared with histopathological findings, with microwave thermocoagulation, increased ADC value seemed to derive from collection of extracellular fluid in the outer zone, where acid phosphatase activity was attenuated. CONCLUSION: ADC values in the areas with delayed enhancement of CE-T1WIs were higher than those in non-affected areas, but MR images could not show areas of coagulation within tumors. Clear detection of the boundaries of coagulated areas required contrast enhancement, even at magnetic field strength of 7T.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Imagen de Difusión por Resonancia Magnética / Electrocoagulación / Neoplasias Femorales / Hipertermia Inducida / Microondas Tipo de estudio: Diagnostic_studies Límite: Animals / Humans Idioma: En Revista: Magn Reson Med Sci Asunto de la revista: DIAGNOSTICO POR IMAGEM Año: 2008 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Japón
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Imagen de Difusión por Resonancia Magnética / Electrocoagulación / Neoplasias Femorales / Hipertermia Inducida / Microondas Tipo de estudio: Diagnostic_studies Límite: Animals / Humans Idioma: En Revista: Magn Reson Med Sci Asunto de la revista: DIAGNOSTICO POR IMAGEM Año: 2008 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Japón