Double immunization strategy with a BoHV-4-vectorialized secreted chimeric peptide BVDV-E2/BoHV-1-gD.
Vaccine
; 26(48): 6031-42, 2008 Nov 11.
Article
en En
| MEDLINE
| ID: mdl-18812200
A bovine herpesvirus 4 was isolated from the milk cell fraction of a healthy cow and his full genome cloned as a bacterial artificial chromosome. So cloned viral genome was used as a vector platform to deliver in vitro and in vivo an optimized secreted chimeric peptide obtained by the fusion of the bovine viral diarrhoea virus glycoprotein E2 ectodomain with the bovine herpesvirus 1 glycoprotein D ectodomain. Recombinant virus infected cells robustly expressed and secreted the chimeric peptide into the culture medium and inoculated animals with the recombinant virus successfully responded toward antigens, gE2 and gD. Thus, this work has implications for the development of safe and effective polyvalent vaccines.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Virales
/
Proteínas del Envoltorio Viral
/
Inmunización
/
Herpesvirus Bovino 4
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Virus de la Diarrea Viral Bovina Tipo 1
/
Proteínas Mutantes Quiméricas
Límite:
Animals
Idioma:
En
Revista:
Vaccine
Año:
2008
Tipo del documento:
Article
País de afiliación:
Italia
Pais de publicación:
Países Bajos