A new anti-loxoscelic serum produced against recombinant sphingomyelinase D: results of preclinical trials.
Am J Trop Med Hyg
; 79(3): 463-70, 2008 Sep.
Article
en En
| MEDLINE
| ID: mdl-18784245
Envenomation by Loxosceles species (brown spider) can lead to local dermonecrosis and to serious systemic effects. The main toxic component in the venom of these spiders is sphingomyelinase D (SMase D) and various isoforms of this toxin are present in Loxosceles venoms. We have produced a new anti-loxoscelic serum by immunizing horses with recombinant SMase D. In the present study, we compared the neutralization efficacy of the new anti-loxoscelic serum and anti-arachnidic serum (the latter serum is used for therapy for loxoscelism in Brazil) against the toxic effects of venoms from spiders of the genus Loxosceles. Neutralization tests showed that anti-SMase D serum has a higher activity against toxic effects of L. intermedia and L. laeta venoms and similar or slightly weaker activity against toxic effects of L. gaucho than that of Arachnidic serum. These results demonstrate that recombinant SMase D can replace venom for anti-venom production and therapy.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Picaduras de Arañas
/
Venenos de Araña
/
Antivenenos
/
Hidrolasas Diéster Fosfóricas
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Am J Trop Med Hyg
Año:
2008
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Estados Unidos