The cloning and expression of alpha-tubulin in Monochamus alternatus.
Insect Mol Biol
; 17(5): 495-504, 2008 Sep.
Article
en En
| MEDLINE
| ID: mdl-18754809
The Japanese pine sawyer Monochamus alternatus is one of the major forest pests. It damages pine directly and transfers the nematode Bursaphelenchus xylophilus to pine wood; resulting in serious economic losses around the world every year. Alpha-tubulin is one of most important proteins in most species. We cloned a ubiquitously expressed M. alternatus alpha-tubulin gene and analysed its nucleotides and protein structure; its sequence characters are consistent with what have been reported in other insects. The alignment of proteins showed that there is high homology of alpha-tubulin between M. alternatus and other species. Western blot and immunocytochemistry analyses suggested a common epitope of alpha-tubulin between M. alternatus and Strongylcentrotus purpuratus. We also expressed the protein in Escherichia coli for further functional studies.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Tubulina (Proteína)
/
Escarabajos
/
Regulación de la Expresión Génica
Límite:
Animals
Idioma:
En
Revista:
Insect Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2008
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Reino Unido