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Identification of intra-group, inter-individual, and gene-specific variances in mRNA expression profiles in the rheumatoid arthritis synovial membrane.
Huber, René; Hummert, Christian; Gausmann, Ulrike; Pohlers, Dirk; Koczan, Dirk; Guthke, Reinhard; Kinne, Raimund W.
Afiliación
  • Huber R; Department of Orthopedics, Experimental Rheumatology Unit, University Hospital Jena, Waldkrankenhaus Rudolf Elle, Eisenberg, Germany.
Arthritis Res Ther ; 10(4): R98, 2008.
Article en En | MEDLINE | ID: mdl-18721452
INTRODUCTION: Rheumatoid arthritis (RA) is a chronic inflammatory and destructive joint disease characterized by overexpression of pro-inflammatory/pro-destructive genes and other activating genes (for example, proto-oncogenes) in the synovial membrane (SM). The gene expression in disease is often characterized by significant inter-individual variances via specific synchronization/desynchronization of gene expression. To elucidate the contribution of the variance to the pathogenesis of disease, expression variances were tested in SM samples of RA patients, osteoarthritis (OA) patients, and normal controls (NCs). METHOD: Analysis of gene expression in RA, OA, and NC samples was carried out using Affymetrix U133A/B oligonucleotide arrays, and the results were validated by real-time reverse transcription-polymerase chain reaction. For the comparison between RA and NC, 568 genes with significantly different variances in the two groups (P
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Artritis Reumatoide / Membrana Sinovial / Variación Genética / ARN Mensajero / Transducción de Señal / Perfilación de la Expresión Génica Tipo de estudio: Diagnostic_studies / Etiology_studies / Observational_studies / Prognostic_studies Límite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Arthritis Res Ther Asunto de la revista: REUMATOLOGIA Año: 2008 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Artritis Reumatoide / Membrana Sinovial / Variación Genética / ARN Mensajero / Transducción de Señal / Perfilación de la Expresión Génica Tipo de estudio: Diagnostic_studies / Etiology_studies / Observational_studies / Prognostic_studies Límite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Arthritis Res Ther Asunto de la revista: REUMATOLOGIA Año: 2008 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido