[Telomeric oligonucleotide complexes with PGEk protein vector: internalization by target cells and antiproliferative properties].
Mol Biol (Mosk)
; 42(2): 286-94, 2008.
Article
en Ru
| MEDLINE
| ID: mdl-18610837
The recombinant protein PGEk, containing residual of the human epidermal factor (hEGF) bearing DNA binding sequence, retains ability of hEGF to bind with hEGF receptor and to induce cell proliferation was shown. On an example of PGEk complexes with telomeric mimic-oligodeoxyribonucleotide d(TTAGGG)4 and with its thio-analogue we had found such systems can be effectively and selectively internalized by hEGF receptors super expressing cells. The association of this process with a protein/oligonucleotide ratio in complexes was investigated. The intracellular localization of oligonucleotides was explored. We had shown that PGEk not only promotes intensive delivery of oligonucleotides, but also protects them from degradation by nucleases. The oligonucleotides in composition of complexes have considerably more expressed cytotoxic activity in comparison with free oligonucleotides.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oligonucleótidos
/
Proteínas Recombinantes de Fusión
/
Telómero
/
Citotoxinas
/
Proliferación Celular
/
Proteínas de Unión al ADN
/
Factor de Crecimiento Epidérmico
Límite:
Animals
/
Humans
Idioma:
Ru
Revista:
Mol Biol (Mosk)
Año:
2008
Tipo del documento:
Article
Pais de publicación:
Rusia