Large-scale production of monoclonal antibodies in suspension culture.
Biotechnol Bioeng
; 32(8): 993-1000, 1988 Oct 05.
Article
en En
| MEDLINE
| ID: mdl-18587817
Monoclonal antibodies are being manufactured for clinical trials in suspension culture at the 1300-L scale. Suspension culture offers some advantages relative to high-density mammalian cell culture methods; in particular, the ability to closely monitor the behavior of cells in a homogeneous environment. Computer control and on-line mass spectrography of exit gases provide instantaneous information about the culture metabolic activity. Air sparging and agitation by marine impeller provide aeration sufficient to maintain a constant dissolved oxygen tension at cell concentrations up to 5.0 x 10(6) cells/mL without causing apparent cell damage.
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Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Biotechnol Bioeng
Año:
1988
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos