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Regulation of protein synthesis at the elongation stage. New insights into the control of gene expression in eukaryotes.
Ryazanov, A G; Rudkin, B B; Spirin, A S.
Afiliación
  • Ryazanov AG; Institute of Protein Research, Academy of Sciences of the USSR, Pushchino, Moscow Region.
FEBS Lett ; 285(2): 170-5, 1991 Jul 22.
Article en En | MEDLINE | ID: mdl-1855586
There are many reports which demonstrate that the rate of protein biosynthesis at the elongation stage is actively regulated in eukaryotic cells. Possible physiological roles for this type of regulation are: the coordination of translation of mRNA with different initiation rate constants; regulation of transition between different physiological states of a cell, such as transition between stages of the cell cycle; and in general, any situation where the maintenance of a particular physiological state is dependent on continuous protein synthesis. A number of covalent modifications of elongation factors offer potential mechanisms for such regulation. Among the various modifications of elongation factors, phosphorylation of eEF-2 by the specific Ca2+calmodulin-dependent eEF-2 kinase is the best studied and perhaps the most important mechanism of regulation of elongation rate. Since this phosphorylation is strictly Ca(2+)-dependent, and makes eEF-2 inactive in translation, this mechanism could explain how changes in the intracellular free Ca2+ concentration may regulate elongation rate. We also discuss some recent findings concerning elongation factors, such as the discovery of developmental stage-specific elongation factors and the regulated binding of eEF-1 alpha to cytoskeletal elements. Together, these observations underline the importance of the elongation stage of translation in the regulation of the cellular processes essential for normal cell life.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Extensión de la Cadena Peptídica de Translación / Biosíntesis de Proteínas / Factores de Elongación de Péptidos / Regulación de la Expresión Génica Límite: Animals Idioma: En Revista: FEBS Lett Año: 1991 Tipo del documento: Article Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Extensión de la Cadena Peptídica de Translación / Biosíntesis de Proteínas / Factores de Elongación de Péptidos / Regulación de la Expresión Génica Límite: Animals Idioma: En Revista: FEBS Lett Año: 1991 Tipo del documento: Article Pais de publicación: Reino Unido