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Improving the prediction accuracy in classification using the combined data sets by ranks of gene expressions.
Kim, Ki-Yeol; Ki, Dong Hyuk; Jeung, Hei-Cheul; Chung, Hyun Cheol; Rha, Sun Young.
Afiliación
  • Kim KY; Oral Cancer Research Institute, Yonsei University College of Dentistry, Seoul, 120-752, South Korea. kky1004@yuhs.ac
BMC Bioinformatics ; 9: 283, 2008 Jun 16.
Article en En | MEDLINE | ID: mdl-18554423
BACKGROUND: The information from different data sets experimented under different conditions may be inconsistent even though they are performed with the same research objectives. More than that, even when the data sets were generated from the same platform, the data agreement may be affected by the technical variation among the laboratories. In this case, it is necessary to use the combined data set after adjusting the differences between such data sets, for detecting the more reliable information. RESULTS: The proposed method combines data sets posterior to the discretization of data sets based on the ranks of the gene expression ratios, and the statistical method is applied to the combined data set for predictive gene selection. The efficiency of the proposed method was evaluated using five colon cancer related data sets, which were experimented using cDNA microarrays with different RNA sources, and one experiment utilized oligonucleotide arrays. NCI-60 cell lines data sets were used, which were performed with two different platforms of cDNA microarrays and Affymetrix HU6800 oligonucleotide arrays. The combined data set by the proposed method predicted the test data sets more accurately than the separated data sets did. The biological significant genes were detected from the combined data set, which were missed on the separated data sets. CONCLUSION: By transforming gene expressions using ranks, the proposed method is not influenced by systematic bias among chips and normalization method. The method may be especially more useful to find predictive genes from data sets which have different scale in gene expressions.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Algoritmos / Transducción de Señal / Análisis de Secuencia por Matrices de Oligonucleótidos / Proteoma / Perfilación de la Expresión Génica / Bases de Datos Genéticas Tipo de estudio: Diagnostic_studies / Prognostic_studies / Risk_factors_studies Idioma: En Revista: BMC Bioinformatics Asunto de la revista: INFORMATICA MEDICA Año: 2008 Tipo del documento: Article País de afiliación: Corea del Sur Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Algoritmos / Transducción de Señal / Análisis de Secuencia por Matrices de Oligonucleótidos / Proteoma / Perfilación de la Expresión Génica / Bases de Datos Genéticas Tipo de estudio: Diagnostic_studies / Prognostic_studies / Risk_factors_studies Idioma: En Revista: BMC Bioinformatics Asunto de la revista: INFORMATICA MEDICA Año: 2008 Tipo del documento: Article País de afiliación: Corea del Sur Pais de publicación: Reino Unido