p53 mutations, ras mutations, and p53-heat shock 70 protein complexes in human lung carcinoma cell lines.

Lehman, T A; Bennett, W P; Metcalf, R A; Welsh, J A; Ecker, J; Modali, R V; Ullrich, S; Romano, J W; Appella, E; Testa, J R

Lehman, T A; Bennett, W P; Metcalf, R A; Welsh, J A; Ecker, J; Modali, R V; Ullrich, S; Romano, J W; Appella, E; Testa, J R.

Afiliación

Lehman TA; Laboratory of Human Carcinogenesis, National Cancer Institute, NIH, Bethesda, Maryland 20892.

Cancer Res ; 51(15): 4090-6, 1991 Aug 01.

Article en En | MEDLINE | ID: mdl-1855224

RESUMEN

The p53 tumor suppressor gene is frequently mutated and the K-ras oncogene is occasionally mutated in primary specimens of human lung carcinomas. These mutated genes also cooperate in the immortalization and neoplastic transformation of rodent cells. To determine whether these mutations are necessary for maintenance of the immortalized and/or neoplastically transformed states of human bronchial epithelial cells, the p53 gene and regions of the ras (K-, H-, and N-) genes were sequenced in nine human lung carcinoma cell lines. Detection of p53 mutations by polymerase chain amplification and direct DNA sequencing was corroborated by p53 immunocytochemistry and coimmunoprecipitation of p53 with heat shock protein 70. p53 and ras genes were frequently, but not always, mutated in the carcinoma cell lines. These data are consistent with the hypothesis that multiple genetic changes involving both protooncogenes and tumor suppressor genes occur during lung carcinogenesis.

Asunto(s)

Genes p53/genética; Genes ras/genética; Proteínas de Choque Térmico/metabolismo; Neoplasias Pulmonares/genética; Mutación/genética; Proteína p53 Supresora de Tumor/metabolismo; Secuencia de Bases; Bronquios/citología; Bronquios/fisiología; Transformación Celular Neoplásica/genética; Células Epiteliales; Exones/fisiología; Humanos; Inmunohistoquímica; Neoplasias Pulmonares/metabolismo; Neoplasias Pulmonares/patología; Datos de Secuencia Molecular; Pruebas de Precipitina; Unión Proteica; Células Tumorales Cultivadas; Proteína p53 Supresora de Tumor/genética

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Genes p53 / Proteína p53 Supresora de Tumor / Genes ras / Proteínas de Choque Térmico / Neoplasias Pulmonares / Mutación Límite: Humans Idioma: En Revista: Cancer Res Año: 1991 Tipo del documento: Article Pais de publicación: Estados Unidos

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