Improved enzyme stability in lipase-catalyzed synthesis of fatty acid ethyl ester from soybean oil.
Appl Biochem Biotechnol
; 152(3): 394-404, 2009 Mar.
Article
en En
| MEDLINE
| ID: mdl-18548204
In this work, we describe the optimization of the ethanolysis of soybean oil by the enzyme Lipozyme TL-IM in the lipase-catalyzed biodiesel synthesis and the improvement of the enzyme stability over repeated batches. The studied process variables were: reaction temperature, substrate molar ratio, enzyme content, and volume of added water. Fractional factorial design was used to analyze the variables so as to select those with higher influence on the reaction and then perform a central composite design to find the optimal reaction conditions. The optimal conditions found were: temperature, 26 degrees C; substrate molar ratio, 7.5:1 (ethanol/oil); enzyme content, 25% in relation to oil weight; and added water, 4% in relation to oil weight. Under these conditions, the yield conversion obtained was 69% in 12 h. The enzyme stability assessment in repeated batches was carried out by washing the immobilized enzyme with different solvents (n-hexane, water, ethanol, and propanol) after each batch. In the treatment with n-hexane, around 80% of the enzyme activity still remains after seven cycles of synthesis, suggesting its economical application on biodiesel production.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Aceite de Soja
/
Ésteres
/
Biocatálisis
/
Ácidos Grasos
/
Lipasa
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Appl Biochem Biotechnol
Año:
2009
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Estados Unidos