Modulation of cultured neural networks using neurotrophin release from hydrogel-coated microelectrode arrays.
J Neural Eng
; 5(2): 203-13, 2008 Jun.
Article
en En
| MEDLINE
| ID: mdl-18477815
Polyacrylamide and poly(ethylene glycol) diacrylate hydrogels were synthesized and characterized for use as drug release and substrates for neuron cell culture. Protein release kinetics was determined by incorporating bovine serum albumin (BSA) into hydrogels during polymerization. To determine if hydrogel incorporation and release affect bioactivity, alkaline phosphatase was incorporated into hydrogels and a released enzyme activity determined using the fluorescence-based ELF-97 assay. Hydrogels were then used to deliver a brain-derived neurotrophic factor (BDNF) from hydrogels polymerized over planar microelectrode arrays (MEAs). Primary hippocampal neurons were cultured on both control and neurotrophin-containing hydrogel-coated MEAs. The effect of released BDNF on neurite length and process arborization was investigated using automated image analysis. An increased spontaneous activity as a response to the released BDNF was recorded from the neurons cultured on the top of hydrogel layers. These results demonstrate that proteins of biological interest can be incorporated into hydrogels to modulate development and function of cultured neural networks. These results also set the stage for development of hydrogel-coated neural prosthetic devices for local delivery of various biologically active molecules.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Potenciales de Acción
/
Factor Neurotrófico Derivado del Encéfalo
/
Materiales Biocompatibles Revestidos
/
Microelectrodos
/
Red Nerviosa
Límite:
Animals
Idioma:
En
Revista:
J Neural Eng
Asunto de la revista:
NEUROLOGIA
Año:
2008
Tipo del documento:
Article
Pais de publicación:
Reino Unido