Chromosome number and cytogenetics of Euphorbia heterophylla L.
Genet Mol Res
; 7(1): 217-22, 2008 Mar 04.
Article
en En
| MEDLINE
| ID: mdl-18393225
Euphorbia heterophylla L. (Euphorbiaceae) is a herbaceous species of great economic importance due to its invasive potential and consequent damage to agriculture and pasture land. For the first time, we provide information on its chromosome number, morphology, and behavior of mitotic chromosomes. Seeds were germinated and submitted to four treatments to obtain metaphases: 0.5% colchicine for 2 to 5 h, at ambient temperature; 0.5% colchicine for 16 to 24 h; 0.0029 M 8-hydroxyquinoline (8-HQ) for 2 to 5 h at ambient temperature, and 0.0029 M 8-HQ for 16 to 24 h at 4 degrees C. The material was then fixed in methanol:acetic acid (3:1) and kept at -20 degrees C for 24 h. Roots were macerated in the enzyme solution of Flaxzyme (NOVO FERMENT)-distilled water (1:40) at 34 degrees C for 2 h and later fixed again. Chromosome preparations were obtained by the dissociation of the apical meristems. The best chromosome preparations were obtained with the use of 8-HQ for 21 h 30 min at 4 degrees C. E. heterophylla showed 2n = 28 chromosomes. The short arm of the largest pair of chromosomes of the complement (pair number 1) displayed a secondary constriction while the nucleolus was observed in the interphasic cell. Structural rearrangements were also observed in the E. heterophylla L. genome. The genomic instability associated with polyploidy may be the result of selection shaped by environmental adaptations and/or human-induced manipulation through agricultural practices.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Análisis Citogenético
/
Euphorbia
/
Cromosomas de las Plantas
Idioma:
En
Revista:
Genet Mol Res
Asunto de la revista:
BIOLOGIA MOLECULAR
/
GENETICA
Año:
2008
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Brasil