Comparison of in-house-developed ELISA with HPLC techniques for the analysis of atrazine residues.
J Environ Sci Health B
; 43(3): 224-30, 2008.
Article
en En
| MEDLINE
| ID: mdl-18368542
In-house developed ELISA was standardized to monitor atrazine residues in different environmental samples. The standard curve was linear, indicating an increase in log concentration with decrease in absorbance (%B/B(0)=1.075-0.042 Log C; r= -0.966). The middle of the test was at 75 ng/L and the lowest detection limit at 4 ng/L. ELISA significantly correlated with the high performance liquid chromatography (HPLC) (r=0.990). Internal validation showed good accuracy and precision. Maximum atrazine residues were present in Jehlum River water/sediments and maize/sugarcane plant roots. Most of the food samples were found to be contaminated. ELISA required less clean-up steps than HPLC, but showed matrix effect in soil/colored extracts.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Atrazina
/
Ensayo de Inmunoadsorción Enzimática
/
Residuos de Plaguicidas
/
Cromatografía Líquida de Alta Presión
/
Herbicidas
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
J Environ Sci Health B
Año:
2008
Tipo del documento:
Article
País de afiliación:
Pakistán
Pais de publicación:
Reino Unido