Clonal deletion and autoreactivity in extrathymic CD4-CD8-(double negative) T cell receptor-alpha/beta T cells.
J Immunol
; 147(10): 3314-8, 1991 Nov 15.
Article
en En
| MEDLINE
| ID: mdl-1834736
Peripheral CD4-CD8- (double negative) T cells (DNT cells) that express TCR-alpha/beta (DNT-alpha/beta cells) are expanded in some autoimmunity-prone mice, but in normal mice these cells have not been extensively studied. In this study, we isolated splenic DNT-alpha/beta cells that are Thy-1+, Ly-1+, MAC-1-, J11d-, and Ia-. Only 5% of these cells were B220+. Freshly isolated DNT-alpha/beta cells did not proliferate in MLC; however, these cells responded strongly to stimulation with matrix-bound anti-TCR-alpha/beta antibodies or Con A. After activation the cells became CD4+CD8-, acquired high reactivity in both syngeneic and allogeneic MLC, but lacked cytotoxicity. After activation the cells gradually reverted to a double-negative phenotype in culture. Surprisingly, despite high autoreactivity the analysis of TCR V beta expression revealed a near normal pattern of Mls-1a- and I-E-induced clonal deletions. These findings reveal that DNT-alpha/beta cells are subject to negative selection and that autoreactivity cannot be attributed to a general failure of clonal deletion in this T cell subpopulation. DNT-alpha/beta cells may normally remain tolerant by down-regulating the CD4 molecule.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Subgrupos de Linfocitos T
/
Receptores de Antígenos de Linfocitos T alfa-beta
Límite:
Animals
Idioma:
En
Revista:
J Immunol
Año:
1991
Tipo del documento:
Article
País de afiliación:
Canadá
Pais de publicación:
Estados Unidos