Clinical significance of LAMB3 and COL7A1 mRNA in esophageal squamous cell carcinoma.

Kita, Y; Mimori, K; Tanaka, F; Matsumoto, T; Haraguchi, N; Ishikawa, K; Matsuzaki, S; Fukuyoshi, Y; Inoue, H; Natsugoe, S; Aikou, T; Mori, M

Kita, Y; Mimori, K; Tanaka, F; Matsumoto, T; Haraguchi, N; Ishikawa, K; Matsuzaki, S; Fukuyoshi, Y; Inoue, H; Natsugoe, S; Aikou, T; Mori, M.

Afiliación

Kita Y; Department of Surgery, Medical Institute of Bioregulation, Kyushu University, Tsurumihara, Beppu, Japan.

Eur J Surg Oncol ; 35(1): 52-8, 2009 Jan.

Article en En | MEDLINE | ID: mdl-18331784

RESUMEN

AIMS: LAMB3 and COL7A1 genes code for the laminin-5beta3 chain and type VII collagen, respectively. They constitute the major components of the basement membrane zone. The aim of the current study was to clarify the clinical significance of LAMB3 and COL7A1 mRNA expression in esophageal squamous cell carcinoma (ESC). METHODS: We quantitated the expression of LAMB3 mRNA and COL7A1 mRNA in malignant esophageal tissues (T) and corresponding normal tissues (N) by real-time quantitative reverse transcription-polymerase chain reaction assays. The clinicopathologic significance of LAMB3 and COL7A1 expression was also determined. Paired T and N tissues were obtained from 66 patients who underwent curative esophagectomy. RESULTS: The expression levels of LAMB3 and COL7A1 mRNAs were higher in malignant tissues than in the corresponding normal tissues. The level of LAMB3 expression was significantly correlated with the depth of invasion and venous invasion (p=0.007 and 0.001, respectively). COL7A1 expression was significantly correlated with depth of tumor invasion and lymphatic invasion (p=0.046, 0.013, respectively). The five-year survival rate was better in the 22 patients with relatively low expression of both LAMB3 and COL7A1 in comparison with the other 44 cases (p<0.05). CONCLUSION: The evaluation of LAMB3 and COL7A1 mRNA expression is useful for predicting the malignant properties of ESC and may prove valuable in predicting the future course of the disease.

Asunto(s)

Carcinoma de Células Escamosas/genética; Moléculas de Adhesión Celular/genética; Colágeno Tipo VIII/genética; Neoplasias Esofágicas/genética; ARN Mensajero/genética; Anciano; Anciano de 80 o más Años; Western Blotting; Carcinoma de Células Escamosas/patología; Moléculas de Adhesión Celular/metabolismo; Distribución de Chi-Cuadrado; Colágeno Tipo VIII/metabolismo; Neoplasias Esofágicas/patología; Femenino; Humanos; Técnicas para Inmunoenzimas; Modelos Lineales; Metástasis Linfática; Masculino; Persona de Mediana Edad; Invasividad Neoplásica/genética; Pronóstico; ARN Mensajero/metabolismo; Reacción en Cadena de la Polimerasa de Transcriptasa Inversa; Kalinina

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN Mensajero / Neoplasias Esofágicas / Carcinoma de Células Escamosas / Moléculas de Adhesión Celular / Colágeno Tipo VIII Tipo de estudio: Prognostic_studies Límite: Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Eur J Surg Oncol Asunto de la revista: NEOPLASIAS Año: 2009 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido

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