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Next generation adoptive immunotherapy--human T cells as carriers of therapeutic nanoparticles.
Mortensen, M W; Kahns, L; Hansen, T; Sorensen, P G; Björkdahl, O; Jensen, M R; Gundersen, H J G; Bjørnholm, T.
Afiliación
  • Mortensen MW; Nano-Science Center, University of Copenhagen, Universitetsparken 5, DK-2100 Copenhagen, Denmark.
J Nanosci Nanotechnol ; 7(12): 4575-80, 2007 Dec.
Article en En | MEDLINE | ID: mdl-18283847
An important step in adoptive immunotherapy in general and specifically with respect to cancer treatment is the initiation of an inflammatory T cell response at the tumor site. Here we suggest a new concept for a controlled inflammatory response in which the intrinsic cytotoxic properties of T cells are upgraded with the properties of nanoparticles transfected into the T cells during the ex vivo expansion process. We report in vitro upgrading of human T cells using PEGylated boron carbide nanoparticles functionalised with a translocation peptide aimed at Boron Neutron Capture Therapy (BNCT). A key finding is that the metabolism of such upgraded human T cells were not affected by a payload of 0.13 pg boron per cell and that the nanoparticles were retained in the cell population after several cell divisions. This is vital for transporting nanoparticles by T cells to the tumor site.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Portadores de Fármacos / Linfocitos T / Inmunoterapia Adoptiva / Nanopartículas Límite: Humans Idioma: En Revista: J Nanosci Nanotechnol Año: 2007 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Estados Unidos
Buscar en Google
Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Portadores de Fármacos / Linfocitos T / Inmunoterapia Adoptiva / Nanopartículas Límite: Humans Idioma: En Revista: J Nanosci Nanotechnol Año: 2007 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Estados Unidos