Microelectrode array (MEA) platform for targeted neuronal transfection and recording.
IEEE Trans Biomed Eng
; 55(2 Pt 2): 827-32, 2008 Feb.
Article
en En
| MEDLINE
| ID: mdl-18270028
Techniques used for nonviral gene transfection often have poor spatial resolution. In this letter, we present a microelectrode array (MEA) system that can precisely transfect exogenous molecules into targeted primary neurons using microelectroporation. An optimal cathodic pulse 4 V in amplitude and 1 ms in duration resulted in a transfection efficiency of 56% and a viability of 82%. Finally, siRNA molecules were transfected into targeted neurons in culture using the aforementioned system.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Transfección
/
Microelectrodos
/
Neuronas
Límite:
Animals
Idioma:
En
Revista:
IEEE Trans Biomed Eng
Año:
2008
Tipo del documento:
Article
Pais de publicación:
Estados Unidos