Culture of subconfluent human fibroblasts and keratinocytes using biodegradable transfer membranes.
Burns
; 34(5): 655-63, 2008 Aug.
Article
en En
| MEDLINE
| ID: mdl-18226463
This study aims to assess the suitability of biodegradable membranes as transfer matrix materials for the culture of subconfluent fibroblasts and keratinocytes. The materials investigated were based on collagen, chitosan and enzyme-digestible cellulose. The proliferation and growth behaviour of human keratinocytes and dermal fibroblasts were analysed and morphology and distribution determined. Cultured fibroblasts exhibited no significant differences in proliferation for the different membrane types, whereas keratinocytes revealed significantly higher proliferation on collagen membranes compared with membranes based on cellulose and chitosan. Co-cultured fibroblasts and keratinocytes from the same donor on collagen membranes showed more homogenous cell distribution, but they segregated in heterologous co-cultures; this effect must be further investigated. Thus, collagen and collagen-coated chitosan membranes are suitable for the subconfluent transfer of human fibroblasts and keratinocytes.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Piel
/
Materiales Biocompatibles
/
Queratinocitos
/
Fibroblastos
/
Membranas Artificiales
Tipo de estudio:
Evaluation_studies
Límite:
Humans
Idioma:
En
Revista:
Burns
Año:
2008
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
Países Bajos